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siRNA expression

In most cases, the shRNA-encoding DNA fragment is made of two chemically synthesized 50- to 70-nucleotide-long oligonucleotides that are annealed and then ligated into a vector or assembled into a virus delivery system. This is a relatively straightforward method, although the synthesis of such long oligonucleotides is error prone.

Vectors with mammalian promoter

Feature: transfection required (therefore mostly suitable for adherent cells and transient expression)

U6 promoter based

H1 promotor based

Viruse based siRNA expression

Adenovirus Non-intergrating

Genome intergrating (long lasting expression)

Lentivirus based shRNA expression

Public accessable virus based shRNA libraries

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